Qalabka fayriska 'Viral DNA / RNA Viral' (Safka)

Qalabka fayriska 'Viral DNA / RNA Viral' (Safka)

Sharax Gaaban:


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Calaamadaha alaabta

Qalabka Sifeynta DNA waxaa loo isticmaalaa soo-saarista habboon, deg-degga ah ee lagu kalsoonaan karo ee DNA-da wareegga bilaashka ah (CFC-DNA) laga bilaabo 1 mL illaa 4 mL plasma / serum. Habka isdaahirinta wuxuu ku saleysan yahay shaxanka shaybaarka iyo wuxuu adeegsanayaa cusbaynta Sigma ee lahaanshaha si loo kala saaro jaantuska. Xirmada waxaa loo isticmaali karaa in lagu kala sooco CFC-DNA oo cabirro kala duwan leh oo laga soo qaado shaybaarka cusub ee plasma / serum. Intaa waxaa dheer, mugga cirifka ayaa si dabacsan loogu hagaajin karaa 25 μl ilaa 50 μl. Plasma-ka la daahiriyey / serum CFC-DNA ayaa lagu xoojin doonaa kor u qaadista si ay ula jaanqaado codsiyada hoose, oo ay ku jiraan PCR, waqtiga dhabta ah ee loo yaqaan 'PCR', PCR-ga methylation iyo falanqaynta xinjirta Koonfurta, microarray iyo NGS.

Wareegga DNA-da ee cabbirada kala duwan ayaa laga sooci karaa samballada dhiigga
Fayraska iyo bakteeriyada DNA ayaa lakala saari karaa
Ku habboon dhammaan noocyada plasma iyo cabbirka cabbirka cabbirka (1 mL ~ 4 mL)
Mugga elution waxaa lagu beddeli karaa furfurmi kala duwan ee 50 μL ~ 100 μL in ay xoogga saaraan wareegga DNA
DNA-da wareega ah ee bilaashka ah ee aan lahayn wax ka-hortage ah ayaa go'doomi kara
DNA tayo sare leh ayaa lagu sifeyn karaa 40-45 daqiiqo
La jaanqaadi kara Tubbooyinka DNA-da ee Bilaashka ah ee 'Streck Cell'

Codsiga

PCR
qPCR
Daabacaadda Koonfurta
Methylation PCR xasaasi ah
CpG diyaarin
Xaddidaadda dheef-shiidka enzyme
Ogaanshaha feyraska
Ogaanshaha bakteeriyada
microarray
NGS


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